Fig. 1

Schematic presentation of constructs used for generation of pET19b plasmids and T7- driven expression of NDV proteins in Rosettagami E. coli cultures. All sketches show the protein C-terminus. a Design of full-length expression of the nucleocapsid gene of NDV strain LaSota, with N-terminal hexahistidin ([H] 6) and AVI tags. b Expression of truncated and frame-edited versions of the P gene expressing unique C-termini of the two P gene-derived proteins P and V as fusion proteins with the heptamerization domain of the human C4 binding protein (C4BP), and tags. An SGS-linker separates virus-specific from fused protein sequences. c Expression cassette of C-terminally truncated versions of the NP protein of NDV LaSota or APMV-8, respectively