MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection

Table 1 Comparison of qRT-PCR and high-throughput sequencing results

MicroRNAs	Organs	qRT-PCR fold-change (Infected/control)	High-throughput sequencing fold-change (Infected/control)
miR-10b	Lung	+2.78	+8.04
miR-novel-chr16_17559	Lung	+2.13	+2.50
miR-novel-GL892871-2_41708	Lung	+2.72	+4.27
miR-26a	Lung	+2.73	+7.36
miR-486	Lung	+2.40	+2.41
miR-24-3p	Lung	−0.55	−0.02
miR-195	Lung	−0.29	−0.02
miR-486	Thymus	+3.30	+2.74
miR-novel-chr16_17559	Thymus	+12.76	+9.35
miR-novel-chr2_21624	Thymus	+4.59	+6.68
miR-19b	Thymus	−0.48	−0.18
miR-486	Kidney	+2.15	+2.59
miR-novel-chr2_21624	Kidney	−0.13	−0.27
let-7f	Spleen	+3.97	+2.77
miR-24-3p	Spleen	+14.66	+17.77
miR-146b	Spleen	−0.38	−0.38
miR-19b	liver	−0.13	−0.044
miR-novel-chr12_7961	liver	−0.18	−0.48
miR-novel-chr2_21624	liver	−0.47	−0.43

“+” and “–” indicate upregulated and downregulated miRNAs, respectively. The fold change cutoffs of the upregulated miRNAs and the downregulated miRNAs were +2 and −0.5, respectively. The qRT-PCR Ct threshold is 0.015

ISSN: 1743-422X