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Table 1 Comparison of qRT-PCR and high-throughput sequencing results

From: MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection

MicroRNAs Organs qRT-PCR fold-change (Infected/control) High-throughput sequencing fold-change (Infected/control)
miR-10b Lung +2.78 +8.04
miR-novel-chr16_17559 Lung +2.13 +2.50
miR-novel-GL892871-2_41708 Lung +2.72 +4.27
miR-26a Lung +2.73 +7.36
miR-486 Lung +2.40 +2.41
miR-24-3p Lung −0.55 −0.02
miR-195 Lung −0.29 −0.02
miR-486 Thymus +3.30 +2.74
miR-novel-chr16_17559 Thymus +12.76 +9.35
miR-novel-chr2_21624 Thymus +4.59 +6.68
miR-19b Thymus −0.48 −0.18
miR-486 Kidney +2.15 +2.59
miR-novel-chr2_21624 Kidney −0.13 −0.27
let-7f Spleen +3.97 +2.77
miR-24-3p Spleen +14.66 +17.77
miR-146b Spleen −0.38 −0.38
miR-19b liver −0.13 −0.044
miR-novel-chr12_7961 liver −0.18 −0.48
miR-novel-chr2_21624 liver −0.47 −0.43
  1. “+” and “–” indicate upregulated and downregulated miRNAs, respectively. The fold change cutoffs of the upregulated miRNAs and the downregulated miRNAs were +2 and −0.5, respectively. The qRT-PCR Ct threshold is 0.015