Table 9 Percentages of HA and NA genes identified in nasal swab supernatants with M gene Cq-value <35, either in low throughput analyses (routine diagnosis performed by the French NRL, January 2014–August 2016) or in high-throughput analyses (longitudinal survey in three herds), according to the M gene Cq-values
From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis
Low-throughput analysesa | High-throughput analysesb | ||||||
|---|---|---|---|---|---|---|---|
Range of M gene Cq-values | Number of samples | Proportion of HA identified | Proportion of NA identified | Range of M gene Cq-values | Number of samples | Proportion of HA identified | Proportion of NA identified |
30 ≤ Cq < 35 | 87 | 52.88% | 20.56% | 30 ≤ Cq < 33 | 189 | 12.17% | 33.86% |
25 ≤ Cq < 30 | 151 | 93.22% | 79.19% | 25 ≤ Cq < 30 | 273 | 89.38% | 96.34% |
20 ≤ Cq < 25 | 198 | 97.00% | 99.14% | 20 ≤ Cq < 25 | 350 | 98.86% | 100% |
< 20 | 106 | 100% | 99.22% | < 20 | 106 | 100% | 100% |