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Table 7 Analytical sensitivity of the real-time RT-PCRs for M, H1av, H1hu, H1huΔ146–147, H1pdm, H3, N1, N1pdm and N2 on ten-fold dilutions of RNA extracted from swIAVs of different subtypes when run on LightCycler®1536

From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis

Virus strain Virus stock dilution M gene RT-qPCR (Cq-value) Real-time RT-PCR for molecular subtyping (Cq-value)
Lineage Name
H1av H1hu H1huΔ146–147 H1pdm H3 N1 N1pdm N2
H1avN1 A/Sw/Cotes d’Armor/0388/2009 10−1 12.01 12.53 no Cq no Cq no Cq no Cq 12.33 no Cq no Cq
10−2 14.90 15.80 no Cq no Cq no Cq no Cq 16.02 no Cq no Cq
10−3 19.46 19.84 no Cq no Cq no Cq no Cq 20.63 no Cq no Cq
10−4 22.83 23.14 no Cq no Cq no Cq no Cq 23.77 no Cq no Cq
10−5 26.53 26.85 no Cq no Cq no Cq no Cq 27.11 no Cq no Cq
10−6 29.52 29.72 no Cq no Cq no Cq no Cq 30.24 no Cq no Cq
10−7 32.81 32.51* no Cq no Cq no Cq no Cq 33.74* no Cq no Cq
10−8 35.45 no Cq no Cq no Cq no Cq no Cq 35.34* no Cq no Cq
H1avN2 A/Sw/Cotes d’Armor/0186/2010 10−1 15.01 15.14 no Cq no Cq no Cq no Cq no Cq no Cq 14.32
10−2 18.14 18.43 no Cq no Cq no Cq no Cq no Cq no Cq 18.04
10−3 21.72 21.96 no Cq no Cq no Cq no Cq no Cq no Cq 22.34
10−4 25.34 25.27 no Cq no Cq no Cq no Cq no Cq no Cq 26.12
10−5 28.91 27.95 no Cq no Cq no Cq no Cq no Cq no Cq 29.51
10−6 32.76 31.62 no Cq no Cq no Cq no Cq no Cq no Cq 32.40
10−7 no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq 34.31*
10−8 no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq
H1huN2 A/Sw/Cotes d’Armor/0113/2006 10−1 14.52 no Cq 16.11 no Cq no Cq no Cq no Cq no Cq 13.71
10−2 17.45 no Cq 19.33 no Cq no Cq no Cq no Cq no Cq 17.68
10−3 22.48 no Cq 23.92 no Cq no Cq no Cq no Cq no Cq 22.71
10−4 25.87 no Cq 27.60 no Cq no Cq no Cq no Cq no Cq 26.40
10−5 28.49 no Cq 30.10* no Cq no Cq no Cq no Cq no Cq 28.89
10−6 33.49 no Cq 33.58* no Cq no Cq no Cq no Cq no Cq 32.04*
10−7 no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq 34.76
10−8 no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq
H1huN2Δ146–147 A/Sw/France/22–130212/2013 10−1 8.59 no Cq 11.90 10.61 no Cq no Cq no Cq no Cq 8.58
10−2 12.08 no Cq 15.01 13.69 no Cq no Cq no Cq no Cq 13.32
10−3 15.54 no Cq 18.64 18.07 no Cq no Cq no Cq no Cq 16.69
10−4 19.05 no Cq 22.40 21.91 no Cq no Cq no Cq no Cq 20.91
10−5 22.85 no Cq 25.88 26.00 no Cq no Cq no Cq no Cq 24.98*
10−6 25.44 no Cq 29.72 29.21 no Cq no Cq no Cq no Cq 27.95
10−7 29.78 no Cq 32.37* 32.17* no Cq no Cq no Cq no Cq 31.09
10−8 32.73 no Cq no Cq 36.31 no Cq no Cq no Cq no Cq 34.43
H1huN1 A/Sw/Cotes d’Armor/0070/2010 10−1 14.85 no Cq 18.39 no Cq no Cq no Cq 16.85 no Cq no Cq
10−2 18.11 no Cq 21.66 no Cq no Cq no Cq 20.45 no Cq no Cq
10−3 22.90 no Cq 26.08 no Cq no Cq no Cq 25.07 no Cq no Cq
10−4 25.71 no Cq 28.73 no Cq no Cq no Cq 27.57 no Cq no Cq
10−5 29.68 no Cq 31.39 no Cq no Cq no Cq 30.95 no Cq no Cq
10−6 no Cq no Cq no Cq no Cq no Cq no Cq 34.42 no Cq no Cq
10−7 33.47* no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq
10−8 No Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq
H1huN1Δ147 A/Sw/Cotes d’Armor/0190/2006 10−1 13.49 no Cq 16.90 no Cq no Cq no Cq 16.07 no Cq no Cq
10−2 17.24 no Cq 20.87 no Cq no Cq no Cq 19.93 no Cq no Cq
10−3 20.86 no Cq 23.92 no Cq no Cq no Cq 23.79 no Cq no Cq
10−4 25.01 no Cq 28.30 no Cq no Cq no Cq 27.43 no Cq no Cq
10−5 28.21 no Cq no Cq no Cq no Cq no Cq 30.37 no Cq no Cq
10−6 31.31 no Cq no Cq no Cq no Cq no Cq 34.50* no Cq no Cq
10−7 no Cq no Cq no Cq no Cq no Cq no Cq 35.28* no Cq no Cq
10−8 no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq
H1N1pdm A/Sw/Sarthe/0255/2010 10−1 13.31 no Cq no Cq no Cq 19.30 no Cq 17.24 16.38 no Cq
10−2 16.62 no Cq no Cq no Cq 22.49 no Cq 21.07 20.55 no Cq
10−3 20.44 no Cq no Cq no Cq 26.63 no Cq 24.76 23.80 no Cq
10−4 24.65 no Cq no Cq no Cq 30.63 no Cq 28.82 27.93 no Cq
10−5 27.94 no Cq no Cq no Cq 33.24 no Cq 32.08 30.39 no Cq
10−6 31.88 no Cq no Cq no Cq 36.13* no Cq 33.85* 34.77 no Cq
10−7 33.50* no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq
10−8 no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq no Cq
H3N2 A/Sw/Flandres/1/1998 10−1 12.96 no Cq no Cq no Cq no Cq 13.28 no Cq no Cq 13.23
10−2 16.61 no Cq no Cq no Cq no Cq 17.15 no Cq no Cq 16.51
10−3 20.88 no Cq no Cq no Cq no Cq 21.32 no Cq no Cq 21.98
10−4 24.05 no Cq no Cq no Cq no Cq 24.44 no Cq no Cq 25.42
10−5 27.57 no Cq no Cq no Cq no Cq 28.15 no Cq no Cq 28.67
10−6 30.48 no Cq no Cq no Cq no Cq 32.06 no Cq no Cq 32.54
10−7 34.01* no Cq no Cq no Cq no Cq 33.74 no Cq no Cq no Cq
10−8 35.31* no Cq no Cq no Cq no Cq 34.38* no Cq no Cq no Cq
  1. Cq quantification cycle. Cq-values are mean Cq-values between duplicates except numbers indicated with an asterisk that were obtained from a single sample (*). Cq-values highlighted in grey represent detection limits