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Table 7 Analytical sensitivity of the real-time RT-PCRs for M, H1av, H1hu, H1huΔ146–147, H1pdm, H3, N1, N1pdm and N2 on ten-fold dilutions of RNA extracted from swIAVs of different subtypes when run on LightCycler®1536

From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis

Virus strain

Virus stock dilution

M gene RT-qPCR (Cq-value)

Real-time RT-PCR for molecular subtyping (Cq-value)

Lineage

Name

H1av

H1hu

H1huΔ146–147

H1pdm

H3

N1

N1pdm

N2

H1avN1

A/Sw/Cotes d’Armor/0388/2009

10−1

12.01

12.53

no Cq

no Cq

no Cq

no Cq

12.33

no Cq

no Cq

10−2

14.90

15.80

no Cq

no Cq

no Cq

no Cq

16.02

no Cq

no Cq

10−3

19.46

19.84

no Cq

no Cq

no Cq

no Cq

20.63

no Cq

no Cq

10−4

22.83

23.14

no Cq

no Cq

no Cq

no Cq

23.77

no Cq

no Cq

10−5

26.53

26.85

no Cq

no Cq

no Cq

no Cq

27.11

no Cq

no Cq

10−6

29.52

29.72

no Cq

no Cq

no Cq

no Cq

30.24

no Cq

no Cq

10−7

32.81

32.51*

no Cq

no Cq

no Cq

no Cq

33.74*

no Cq

no Cq

10−8

35.45

no Cq

no Cq

no Cq

no Cq

no Cq

35.34*

no Cq

no Cq

H1avN2

A/Sw/Cotes d’Armor/0186/2010

10−1

15.01

15.14

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

14.32

10−2

18.14

18.43

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

18.04

10−3

21.72

21.96

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

22.34

10−4

25.34

25.27

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

26.12

10−5

28.91

27.95

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

29.51

10−6

32.76

31.62

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

32.40

10−7

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

34.31*

10−8

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

H1huN2

A/Sw/Cotes d’Armor/0113/2006

10−1

14.52

no Cq

16.11

no Cq

no Cq

no Cq

no Cq

no Cq

13.71

10−2

17.45

no Cq

19.33

no Cq

no Cq

no Cq

no Cq

no Cq

17.68

10−3

22.48

no Cq

23.92

no Cq

no Cq

no Cq

no Cq

no Cq

22.71

10−4

25.87

no Cq

27.60

no Cq

no Cq

no Cq

no Cq

no Cq

26.40

10−5

28.49

no Cq

30.10*

no Cq

no Cq

no Cq

no Cq

no Cq

28.89

10−6

33.49

no Cq

33.58*

no Cq

no Cq

no Cq

no Cq

no Cq

32.04*

10−7

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

34.76

10−8

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

H1huN2Δ146–147

A/Sw/France/22–130212/2013

10−1

8.59

no Cq

11.90

10.61

no Cq

no Cq

no Cq

no Cq

8.58

10−2

12.08

no Cq

15.01

13.69

no Cq

no Cq

no Cq

no Cq

13.32

10−3

15.54

no Cq

18.64

18.07

no Cq

no Cq

no Cq

no Cq

16.69

10−4

19.05

no Cq

22.40

21.91

no Cq

no Cq

no Cq

no Cq

20.91

10−5

22.85

no Cq

25.88

26.00

no Cq

no Cq

no Cq

no Cq

24.98*

10−6

25.44

no Cq

29.72

29.21

no Cq

no Cq

no Cq

no Cq

27.95

10−7

29.78

no Cq

32.37*

32.17*

no Cq

no Cq

no Cq

no Cq

31.09

10−8

32.73

no Cq

no Cq

36.31

no Cq

no Cq

no Cq

no Cq

34.43

H1huN1

A/Sw/Cotes d’Armor/0070/2010

10−1

14.85

no Cq

18.39

no Cq

no Cq

no Cq

16.85

no Cq

no Cq

10−2

18.11

no Cq

21.66

no Cq

no Cq

no Cq

20.45

no Cq

no Cq

10−3

22.90

no Cq

26.08

no Cq

no Cq

no Cq

25.07

no Cq

no Cq

10−4

25.71

no Cq

28.73

no Cq

no Cq

no Cq

27.57

no Cq

no Cq

10−5

29.68

no Cq

31.39

no Cq

no Cq

no Cq

30.95

no Cq

no Cq

10−6

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

34.42

no Cq

no Cq

10−7

33.47*

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

10−8

No Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

H1huN1Δ147

A/Sw/Cotes d’Armor/0190/2006

10−1

13.49

no Cq

16.90

no Cq

no Cq

no Cq

16.07

no Cq

no Cq

10−2

17.24

no Cq

20.87

no Cq

no Cq

no Cq

19.93

no Cq

no Cq

10−3

20.86

no Cq

23.92

no Cq

no Cq

no Cq

23.79

no Cq

no Cq

10−4

25.01

no Cq

28.30

no Cq

no Cq

no Cq

27.43

no Cq

no Cq

10−5

28.21

no Cq

no Cq

no Cq

no Cq

no Cq

30.37

no Cq

no Cq

10−6

31.31

no Cq

no Cq

no Cq

no Cq

no Cq

34.50*

no Cq

no Cq

10−7

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

35.28*

no Cq

no Cq

10−8

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

H1N1pdm

A/Sw/Sarthe/0255/2010

10−1

13.31

no Cq

no Cq

no Cq

19.30

no Cq

17.24

16.38

no Cq

10−2

16.62

no Cq

no Cq

no Cq

22.49

no Cq

21.07

20.55

no Cq

10−3

20.44

no Cq

no Cq

no Cq

26.63

no Cq

24.76

23.80

no Cq

10−4

24.65

no Cq

no Cq

no Cq

30.63

no Cq

28.82

27.93

no Cq

10−5

27.94

no Cq

no Cq

no Cq

33.24

no Cq

32.08

30.39

no Cq

10−6

31.88

no Cq

no Cq

no Cq

36.13*

no Cq

33.85*

34.77

no Cq

10−7

33.50*

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

10−8

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

no Cq

H3N2

A/Sw/Flandres/1/1998

10−1

12.96

no Cq

no Cq

no Cq

no Cq

13.28

no Cq

no Cq

13.23

10−2

16.61

no Cq

no Cq

no Cq

no Cq

17.15

no Cq

no Cq

16.51

10−3

20.88

no Cq

no Cq

no Cq

no Cq

21.32

no Cq

no Cq

21.98

10−4

24.05

no Cq

no Cq

no Cq

no Cq

24.44

no Cq

no Cq

25.42

10−5

27.57

no Cq

no Cq

no Cq

no Cq

28.15

no Cq

no Cq

28.67

10−6

30.48

no Cq

no Cq

no Cq

no Cq

32.06

no Cq

no Cq

32.54

10−7

34.01*

no Cq

no Cq

no Cq

no Cq

33.74

no Cq

no Cq

no Cq

10−8

35.31*

no Cq

no Cq

no Cq

no Cq

34.38*

no Cq

no Cq

no Cq

  1. Cq quantification cycle. Cq-values are mean Cq-values between duplicates except numbers indicated with an asterisk that were obtained from a single sample (*). Cq-values highlighted in grey represent detection limits