From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis
Real-time RT-PCRa for molecular subtyping | RNA extract from (virus strain – name and subtype) | RNA extract dilution (≈ M gene Cq-value) | Mean Cq-value | SD | Inter-assay CV (%) |
---|---|---|---|---|---|
H1av | A/Sw/Cotes d’Armor/0388/2009 (H1avN1) | 10−4 (≈ 23.83) | 24.50 | 0.9 | 1.20 |
H1hu | A/Sw/Scotland/410440/1994 (H1huN2) | 10−3 (≈ 20.43) | 25.63 | 0.39 | 1.52 |
H1hu Δ146–147 | A/Sw/France/22–130212/2013 (H1huN2Δ146–147) | 10−4 (≈ 21.3) | 22.80 | 0.71 | 3.12 |
H3 | A/Sw/Flandres/1/1998 (H3N2) | 10−2 (≈ 16.7) | 18.60 | 0.31 | 1.68 |
N1 | A/Sw/Cotes d’Armor/0388/2009 (H1avN1) | 10−4 (≈ 23.83) | 25.64 | 0.37 | 1.46 |
N2 | A/Sw/Scotland/410440/1994 (H1huN2) | 10−3 (≈ 20.43) | 24.78 | 1.19 | 4.79 |
A/Sw/Flandres/1/1998 (H3N2) | 10−3 (≈ 20.21)* | 28.56 | 1.86 | 6.51 |