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Table 4 Performance of real-time RT-PCRs for H1av, H1hu, H1huΔ146–147, H3, N1 and N2 on ten-fold dilutions of RNA extracted from swIAVs representative of the different subtypes

From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis

Virus strain Criteria Real-time RT-PCRb for molecular subtyping
H1av H1hu H1hu Δ146–147 H3 N1 N2
A/Sw/Cotes d’Armor/0388/2009 (H1avN1) Range of linearitya < 12.52 to 33.36     < 12.52 to 27.03  
Slope −3.2918     −3.226  
R2 0.9986     0.9957  
Efficacy 101.27%     104.16%  
A/Sw/Scotland/410440/1994 (H1huN2) Range of linearitya   <13.47 to 23.79     <13.47 to 23.79
Slope   −3.3483     −3.2725
R2   0.9998     0.9957
Efficacy   98.91%     102.10%
A/Sw/France/22–130212/2013 (H1huN2Δ146–147) Range of linearitya   <10.98 to 25.64 <10.98 to >35.16    <10.98 to 28.06
Slope   −3.281 −3.4008    −3.266
R2   0.9997 0.9992    0.9988
Efficacy   101.74% 96.81%    102.39%
A/Sw/Flandres/1/1998 (H3N2) Range of linearitya     16.7 to 32.7   <13.13 to 23.57
Slope     −3.2964   −3.297
R2     0.9982   0.998
Efficacy     101.08%   101.07%
  1. aThe range of linearity is given as the interval of Cq-values obtained from M gene RT-qPCR on diluted samples (Thermocycler MxPro – Mx3005P). R2: coefficient of linear regression. bHA RT-qPCRs were run as simplex assays; N1 and N2 RT-qPCRs were run in duplex