From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis
Virus strain | Criteria | Real-time RT-PCRb for molecular subtyping | |||||
---|---|---|---|---|---|---|---|
H1av | H1hu | H1hu Δ146–147 | H3 | N1 | N2 | ||
A/Sw/Cotes d’Armor/0388/2009 (H1avN1) | Range of linearitya | < 12.52 to 33.36 | < 12.52 to 27.03 | ||||
Slope | −3.2918 | −3.226 | |||||
R2 | 0.9986 | 0.9957 | |||||
Efficacy | 101.27% | 104.16% | |||||
A/Sw/Scotland/410440/1994 (H1huN2) | Range of linearitya | <13.47 to 23.79 | <13.47 to 23.79 | ||||
Slope | −3.3483 | −3.2725 | |||||
R2 | 0.9998 | 0.9957 | |||||
Efficacy | 98.91% | 102.10% | |||||
A/Sw/France/22–130212/2013 (H1huN2Δ146–147) | Range of linearitya | <10.98 to 25.64 | <10.98 to >35.16 | <10.98 to 28.06 | |||
Slope | −3.281 | −3.4008 | −3.266 | ||||
R2 | 0.9997 | 0.9992 | 0.9988 | ||||
Efficacy | 101.74% | 96.81% | 102.39% | ||||
A/Sw/Flandres/1/1998 (H3N2) | Range of linearitya | 16.7 to 32.7 | <13.13 to 23.57 | ||||
Slope | −3.2964 | −3.297 | |||||
R2 | 0.9982 | 0.998 | |||||
Efficacy | 101.08% | 101.07% |