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Table 4 Performance of real-time RT-PCRs for H1av, H1hu, H1huΔ146–147, H3, N1 and N2 on ten-fold dilutions of RNA extracted from swIAVs representative of the different subtypes

From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis

Virus strain

Criteria

Real-time RT-PCRb for molecular subtyping

H1av

H1hu

H1hu Δ146–147

H3

N1

N2

A/Sw/Cotes d’Armor/0388/2009 (H1avN1)

Range of linearitya

< 12.52 to 33.36

   

< 12.52 to 27.03

 

Slope

−3.2918

   

−3.226

 

R2

0.9986

   

0.9957

 

Efficacy

101.27%

   

104.16%

 

A/Sw/Scotland/410440/1994 (H1huN2)

Range of linearitya

 

<13.47 to 23.79

   

<13.47 to 23.79

Slope

 

−3.3483

   

−3.2725

R2

 

0.9998

   

0.9957

Efficacy

 

98.91%

   

102.10%

A/Sw/France/22–130212/2013 (H1huN2Δ146–147)

Range of linearitya

 

<10.98 to 25.64

<10.98 to >35.16

  

<10.98 to 28.06

Slope

 

−3.281

−3.4008

  

−3.266

R2

 

0.9997

0.9992

  

0.9988

Efficacy

 

101.74%

96.81%

  

102.39%

A/Sw/Flandres/1/1998 (H3N2)

Range of linearitya

   

16.7 to 32.7

 

<13.13 to 23.57

Slope

   

−3.2964

 

−3.297

R2

   

0.9982

 

0.998

Efficacy

   

101.08%

 

101.07%

  1. aThe range of linearity is given as the interval of Cq-values obtained from M gene RT-qPCR on diluted samples (Thermocycler MxPro – Mx3005P). R2: coefficient of linear regression. bHA RT-qPCRs were run as simplex assays; N1 and N2 RT-qPCRs were run in duplex