Table 3 Analytical sensitivity of the real-time RT-PCRs for H1av, H1hu, H3, N1 and N2 on ten-fold dilutions of RNA extracted from swIAVs of different subtypes
From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis
Virus strain | Virus stock dilution | M gene RT-qPCRa(Cq-value) | Real-time RT-PCRb for molecular subtyping (Cq-value) | ||||||
|---|---|---|---|---|---|---|---|---|---|
Lineage | Name | ||||||||
H1av | H1hu | H1huΔ146–147 | H3 | N1 | N2 | ||||
H1avN1 | A/Sw/Cotes d’Armor/0388/2009 | 10−1 | 12.52 | 16.10 | no Cq | nt | no Cq | 15.52 | no Cq |
10−2 | 16.09 | 19.52 | no Cq | nt | no Cq | 18.75 | no Cq | ||
10−3 | 20.16 | 22.77 | no Cq | nt | no Cq | 22.46 | no Cq | ||
10−4 | 23.83 | 25.85 | no Cq | nt | no Cq | 25.71 | no Cq | ||
10−5 | 27.03 | 28.86 | no Cq | nt | no Cq | 28.18 | no Cq | ||
10−6 | 29.79 | 32.32 | no Cq | nt | no Cq | no Cq | no Cq | ||
10−7 | 33.36 | 36.26 | no Cq | nt | no Cq | no Cq | no Cq | ||
10−8 | 38.59 | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
10−9 | no Cq | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
10−10 | no Cq | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
H1huN2 | A/Sw/Scotland/410440/1994 | 10−1 | 13.47 | no Cq | 15.18 | nt | no Cq | no Cq | 16.90 |
10−2 | 17.15 | no Cq | 18.53 | nt | no Cq | no Cq | 19.92 | ||
10−3 | 20.43 | no Cq | 21.99 | nt | no Cq | no Cq | 23.54 | ||
10−4 | 23.79 | no Cq | 25.19 | nt | no Cq | no Cq | 26.60 | ||
10−5 | 27.25 | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
10−6 | 30.11 | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
10−7 | 33.05 | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
10−8 | 37.48 | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
10−9 | no Cq | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
10−10 | no Cq | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||
H1huN2Δ146–147 | A/Sw/France/22–130212/2013 | 10−1 | 10.98 | nt | 11.88 | 12.68 | nt | no Cq | 11.48 |
10−2 | 14.67 | nt | 15.26 | 16.10 | nt | no Cq | 14.38 | ||
10−3 | 17.82 | nt | 18.66 | 20.07 | nt | no Cq | 17.78 | ||
10−4 | 21.3 | nt | 21.77 | 22.95 | nt | no Cq | 20.92 | ||
10−5 | 25.64 | nt | 25.03 | 26.53 | nt | no Cq | 24.54 | ||
10−6 | 28.06 | nt | no Cq | 29.83 | nt | no Cq | 33.03* | ||
10−7 | 31.54 | nt | no Cq | 33.36 | nt | no Cq | no Cq | ||
10−8 | 35.16 | nt | no Cq | 37.03 | nt | no Cq | no Cq | ||
10−9 | no Cq | nt | no Cq | 39.63 | nt | no Cq | no Cq | ||
10−10 | no Cq | nt | no Cq | no Cq | nt | no Cq | no Cq | ||
H3N2 | A/Sw/Flandres/1/1998 | 10−1 | 13.13 | no Cq | no Cq | nt | 17.045 | no Cq | 16.865 |
10−2 | 16.7 | no Cq | no Cq | nt | 19.88 | no Cq | 19.725 | ||
10−3 | 20.21 | no Cq | no Cq | nt | 23.55 | no Cq | 23.275 | ||
10−4 | 23.57 | no Cq | no Cq | nt | 27.075 | no Cq | 26.67* | ||
10−5 | 26.9 | no Cq | no Cq | nt | 30.235 | no Cq | no Cq | ||
10−6 | 30.07 | no Cq | no Cq | nt | 33.53 | no Cq | no Cq | ||
10−7 | 32.7 | no Cq | no Cq | nt | 36.335 | no Cq | no Cq | ||
10−8 | 37.04 | no Cq | no Cq | nt | 37.86 | no Cq | no Cq | ||
10−9 | no Cq | no Cq | no Cq | nt | 39.99 | no Cq | no Cq | ||
10−10 | no Cq | no Cq | no Cq | nt | no Cq | no Cq | no Cq | ||