Monitoring of Poyang lake water for sewage contamination using human enteric viruses as an indicator

Table 2 List of PCR primers used in this study with optimal amplification conditions

Virus	Primer name	Primer Sequence (5′ → 3′)	Length (bp)	Amplification condition				Reference
Virus	Primer name	Primer Sequence (5′ → 3′)	Length (bp)	TA	Mg (mM)	Primer (μM)	BSA
NoV GI	QNIF4	CGCTGGATGCGNTTCCAT	8 6	58	2.0	0.4	Yes	Loisy et al., 2005
NoV GI	NV1LCR	CCTTAGACGCCATCATCATTTAC	8 6	58	2.0	0.4	Yes	Loisy et al., 2005
NoV GII	COG2F	CARGARBCNATGTTYAGRTGGATGAG	9 7	58	2.0	0.8	Yes	Kageyama et al., 2003
NoV GII	COG2R	TCGACGCCATCTTCATTCACA	9 7	58	2.0	0.8	Yes	Kageyama et al., 2003
EoV	EQ-1	ACATGGTGTGAAGAGTCTATTGAGCT	1 4 2	60	1.5	0.6	Yes	Dierssen et al., 2008
EoV	EQ-2	CCAAAGTAGTCGGTTCCGC	1 4 2	60	1.5	0.6	Yes	Dierssen et al., 2008
AdV	ADV-F	GCCACGGTGGGGTTTCTAAACTT	1 3 2	54	1.5	0.6	Yes	Gunson et al., 2009
AdV	ADV-R	GCCCCAGTGGTCTTACATGCACATC	1 3 2	54	1.5	0.6	Yes	Gunson et al., 2009

^aNoV GI, human norovirus genotype I; NoV GII, human norovirus genotype II; EoV, human enterovirus; AdV, human adenovirus

ISSN: 1743-422X