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Fig. 5 | Virology Journal

Fig. 5

From: Bacterial ribonuclease binase exerts an intra-cellular anti-viral mode of action targeting viral RNAs in influenza a virus-infected MDCK-II cells

Fig. 5

Impact of binase on the viral expression efficiency of the H1N1pdm09 RNP complex. Effect of binase on the viral expression activity of the H1N1pdm09 RNP complex was analyzed in 293 T cells transfected with plasmids expressing the polymerase subunits (PB1, PB2, and PA) and NP of H1N1pdm09 together with vRNA-like transcript (pPol1-CAT-RT) encoding the CAT reporter gene, was analyzed for CAT activity 48 h post transfection. The cells were treated with 104 and 105 U/ml of binase after transfection. In the control, binase was absent. Statistical significance was investigated using the Student’s t-test. ***P < 0.001

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Virology Journal

ISSN: 1743-422X