TY - JOUR AU - He, Ying AU - Taylor, Tonya L. AU - Dimitrov, Kiril M. AU - Butt, Salman L. AU - Stanton, James B. AU - Goraichuk, Iryna V. AU - Fenton, Heather AU - Poulson, Rebecca AU - Zhang, Jian AU - Brown, Corrie C. AU - Ip, Hon S. AU - Isidoro-Ayza, Marcos AU - Afonso, Claudio L. PY - 2018 DA - 2018/01/12 TI - Whole-genome sequencing of genotype VI Newcastle disease viruses from formalin-fixed paraffin-embedded tissues from wild pigeons reveals continuous evolution and previously unrecognized genetic diversity in the U.S. JO - Virology Journal SP - 9 VL - 15 IS - 1 AB - Newcastle disease viruses (NDV) are highly contagious and cause disease in both wild birds and poultry. A pigeon-adapted variant of genotype VI NDV, often termed pigeon paramyxovirus 1, is commonly isolated from columbids in the United States and worldwide. Complete genomic characterization of these genotype VI viruses circulating in wild columbids in the United States is limited, and due to the genetic variability of the virus, failure of rapid diagnostic detection has been reported. Therefore, in this study, formalin-fixed paraffin-embedded (FFPE) samples were subjected to next-generation sequencing (NGS) to identify and characterize these circulating viruses, providing valuable genetic information. NGS enables multiple samples to be deep-sequenced in parallel. When used on FFPE samples, this methodology allows for retrospective studies of infectious organisms. SN - 1743-422X UR - https://doi.org/10.1186/s12985-017-0914-2 DO - 10.1186/s12985-017-0914-2 ID - He2018 ER -