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Fig. 2 | Virology Journal

Fig. 2

From: Enhanced human enterovirus 71 infection by endocytosis inhibitors reveals multiple entry pathways by enterovirus causing hand-foot-and-mouth diseases

Fig. 2

The effect of CPZ on EV71 infection is dynamin independent. a. Cells were infected by EV71 and treated with 20 μM CPZ or 80 μM DNS for 6 h (Vero, RD, MRC-5 and HEK293T, MOI = 1) or 12 h (Hela, A549, Huh7 and HepG2, MOI = 5). After removal of virus and the drugs, the cells were washed and continued to culture for 6 h or 12 h and then processed for flow cytometry. Means of three experiments with SD are shown. *, p < 0.05. b. A549 and RD cells were pretreated by 20 μM CPZ or 80 μM DNS for 2 h prior to incubation with AlexaFluor 488-conjugated transferrin (25 μg/ml) or AlexaFluor 594-conjugated cholera toxin B (CTxB, 10 μg/ml). After 15 min of incubation, cells were fixed and analyzed by immunofluorescent microscope. Arrow heads showed the bright puncta around nuclei. Scale bar, 100 μm. c. Various cells were infected by EV71 and treated by CPZ (20 μM), DNS (80 μM) or their combinations for 6 h (Vero, RD, MRC-5 and HEK293T, MOI = 1) or 12 h (Hela, A549, Huh7 and HepG2, MOI = 5). After removal of virus and the drug, cells were washed and continued to culture for 6 h or 12 h and then processed for flow cytometry. Means of three experiments with SD are shown. *, p < 0.05

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