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Fig. 1 | Virology Journal

Fig. 1

From: Pulmonary edema following central nervous system lesions induced by a non- mouse-adapted EV71 strain in neonatal BALB/c mice

Fig. 1

ZZ1350 infection led to high cytotoxicity against Vero cells and RD cells. Viral RNA in ZZ1350-infected RD cells was extracted and identified by qRT-PCR (a). Evolutionary tree based on VP1 genome (b) was performed using MEGA software. CPEs and EV71 distribution (c) in Vero cells induced by different doses of ZZ1350 were imaged via light microscopy (amplification: 100×) or confocal microscopy (amplification: 400×) at 24 hpi. The viabilities of Vero cells (d) following virus infection were determined using the MTT assay. EV71 positive area (%) (e) was evaluated using Image-Pro Plus 6.0 software. Cell pellets of normal (f), infected RD cells (g, h) were fixed and investigated with electron microscopy. Data are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3)

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