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Table 2 Prediction of the putative pp1a/pp1b cleavage sites of BatCoV-Ita1/2 based on sequence comparison with MERS-CoV strain HCoV-EMC/2012

From: Detection and full genome characterization of two beta CoV viruses related to Middle East respiratory syndrome from bats in Italy

NSP

Position of the putative cleavage sitesa

Protein size (no. of amino acids)

Putative functional domain(s)b

NSP1

Met1-Gly195

195

 

NSP2

Asn196-Gly855

660

 

NSP3

Ala856-Gly2738

1883

ADRP, PL2pro

NSP4

Ala2739-Gln3245

507

 

NSP5

Ser3246-Gln3551

306

3CLpro

NSP6

Ser3552-Gln3843

292

 

NSP7

Ser3844-Gln3926

83

 

NSP8

Ala3927-Gln4125

199

Primase

NSP9

Asn4126-Gln4235

110

 

NSP10

Ala4236-Gln4375

140

 

NSP11

Ser4376-Ile4389

14

Short peptide at the end of ORF1a

NSP12

Ser4376-Gln5309

934

RdRp

NSP13

Ala5310-Gln5907

598

HEL, NTPase

NSP14

Ser5908-Gln6431

524

ExoN, NMT

NSP15

Gly6432-Gln6773

342

NendoU

NSP16

Ala6774-His7076

303

OMT

  1. aSuperscript numbers indicate positions in polyprotein pp1a/pp1ab or position in available sequence with the supposition of a ribosomal frameshift based on the conserved slippery sequenced (UUUAAAC) of Coronaviruses. Localized at nucleotide position 13,359–13,365 for BatCoV-Ita1 and 13,350–13,356 for BatCoV-Ita2
  2. bADRP ADP-ribose 1-phosphatase, PL2pro papain-like protease 2, 3CLpro coronavirus NSP5 protease, Hel helicase, NTPase nucleoside triphosphatase, ExoN exoribonuclease, NMT N7 methyltransferase, NendoU endoribonuclease, OMT 2’ O-methyltransferase