Table 2 Positive detection rate for each of the two tested assays according to cytological results: normal, atypical squamous cells of undetermined significance (ASCUS), low-grade lesions (LSIL) and high-grade lesions + (HSIL+)
Assay | Normal (n = 50) | ASCUS (n = 12) | LSIL (n = 7) | HSIL+ (n = 14) |
|---|---|---|---|---|
Conventional nested PCR | 24 (48%) | 6 (50%) | 6 (85.71%) | 14 (100%) |
HPV16 | 21 (87.5%) | 4 (66.67) | 4 (66.67%) | 12 (85.71%) |
HPV18 | 5 (20.8%) | 2 (33.33) | 1 (16.67%) | 3 (21.43%) |
Other high-risk genotypes | 15 (62.5%) | 1 (16.67) | 3 (50%) | 4 (28.57%) |
Abbott RealTime | 13 (26%) | 4 (33.33%) | 6 (85.71%) | 14 (100%) |
HPV16 | 7 (53.85%) | 2 (50%) | 2 (33.33) | 10 (71.43%) |
HPV18 | 1 (7.69%) | 2 (50%) | 1 (16.67%) | 2 (14.29%) |
Other high-risk genotypes | 7 (53.85%) | – | 4 (66.67%) | 3 (21.43%) |