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Fig. 3 | Virology Journal

Fig. 3

From: Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA – effects on NS5B catalysis and allosteric inhibition

Fig. 3

Effect of AnxA2 and mAnxA2 on the polymerase activity of NS5B, measured as the de novo synthesis of RNA using RNA-DNA hybrid surfaces and rNTPs. a Illustration of the biosensor-based HCV polymerase assay monitoring the de novo synthesis of RNA using RNA-DNA hybrid surfaces and rNTPs. A biotinylated (b) ssDNA oligo is captured on a biosensor chip via streptavidin (S) immobilized by amine coupling. The 31-mer RNA is subsequently hybridized to the ssDNA. 100 nM NS5B (b and c) and nucleotides are injected over the surface and the polymerase activity monitored in real-time in the presence of 100 nM (b) or 200 nM (c) AnxA2 or mAnxA2 (d) Binding of 100 nM NS5B, 1000 nM AnxA2 and 200 nM mAnxA2 to the sensor surfaces in the absence of rNTPs (negative control experiment)

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