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Fig. 1 | Virology Journal

Fig. 1

From: Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA – effects on NS5B catalysis and allosteric inhibition

Fig. 1

Interactions between AnxA2 and NS5B demonstrated in real-time by SPR biosensor technology. a 31 to 500 nM NS5B injected over immobilized AnxA2 (3595 RU), using an activated and inactivated blank surface as reference. b 63 to 1000 nM AnxA2 injected over immobilized NS5B (2634 RU). c 7.8 to 125 nM AnxA2 injected over immobilized NS5B (12,400 RU). The reference surface contained immobilized NS5B (11,765 RU) inactivated by injections of 6 M guanidine-HCl. d 31 to 500 nM NS5B injected over immobilized mAnxA2 (unable to bind RNA) (1041 RU). An activated and inactivated blank surface was used as reference. Experiments with immobilized AnxA2 wt and mAnxA2 (a and d, respectively) included regeneration of the surface between analyte injections, while experiments with immobilized NS5B (b and c) were performed without regeneration of the surface between injections. e-h Steady state analysis of experiments a-d was based on report points taken at the end of the injection although steady state was not reached in all report points. Vertical lines on steady-state analysis plots indicate KD values (i.e. concentration resulting in 50% saturation)

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