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Fig. 3 | Virology Journal

Fig. 3

From: Hepatitis B virus x protein induces epithelial-mesenchymal transition of hepatocellular carcinoma cells by regulating long non-coding RNA

Fig. 3

ZEB2-AS1 was an important lncRNA that mediated HBx-induced EMT. a The level of ZEB2-AS1 was verified by a real-time PCR assay. The siRNA can effectively inhibit the level of ZEB2-AS1. b Transwell assay. Knockdown of ZEB2-AS1 inhibited the metastatic ability of HepG2 cells. c. The level of ZEB2-AS1 was verified by a real-time PCR assay. ZEB2-AS1 was up-regulated by 3.79-fold by HBx, but compromised by si-ZEB2-AS1 in HepG2-HBx. d and e The regulating effect of ZEB2-AS1 on EMT was assessed by transwell assay. HBx promoted the metastatic ability of HCC cells; however, siRNA treatment against ZEB2-AS1 rescued the phenotype in these cells. f EMT markers were detected by Western blot. HBx overexpression enhanced vimentin and ZEB2 expression, whereas it inhibited E-cadherin expression. However, knockdown ZEB2-AS1 reversed the above protein expression. *pā€‰<ā€‰0.05, **pā€‰<ā€‰0.01

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