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Fig. 2 | Virology Journal

Fig. 2

From: Hepatitis B virus x protein induces epithelial-mesenchymal transition of hepatocellular carcinoma cells by regulating long non-coding RNA

Fig. 2

The effect of HBx on HCC EMT was detected by in vivo and in vitro experiments. a Morphology observation under the inverted microscope. HBx induced morphological alterations from the epithelial phenotype to the mesenchymal phenotype characterized by shuttle shape and scattered growth. b and c Transwell assays for migration and invasion. HBx promoted the metastatic ability and induced more cells to pass through the membrane. d and e The number of transmembrane cells are presented by column diagram. f Lung metastatic tumours in mice. The number of metastatic tumours was significantly higher in the HBx overexpressed HepG2 cells than the control group. g Immunocytochemistry assay for HBx, E-cadherin and vimentin. HBx overexpression in HepG2 cells resulted in decreased of E-cadherin and increased of vimentin. *p < 0.05, **p < 0.01

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