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Fig. 2 | Virology Journal

Fig. 2

From: The CC chemokine ligand (CCL) 1, upregulated by the viral transactivator Tax, can be downregulated by minocycline: possible implications for long-term treatment of HTLV-1-associated myelopathy/tropical spastic paraparesis

Fig. 2

Tax-dependent constitutive expression of CCL1 in Tax-inducible JPX9 cells. RNA and protein expression of CCL1 in JPX9 cells. JPX9 is a Jurkat (HTLV-1-negative human T-cell leukemia cell line) subclone generated by stable transfection of a functional Tax expression-plasmid vector. Tax expression was induced by adding CdCl2 to the culture medium (final concentration: 10 μM). RT-PCR (a) and western blotting (b) showed that treatment of JPX9 cells with CdCl2 induced the expression of Tax at the RNA and protein levels, respectively. c. Flow cytometry data showed that treatment of JPX9 cells with CdCl2 induced CCL1 expression and that CCL1 was expressed exclusively in cells that also expressed Tax. d. ELISA analysis of culture supernatants showed that the addition of CdCl2 to the JPX9 cell culture resulted in an increase in CCL1 expression within 24 h. CCL1 levels were found to reach a peak after 48 h of culture

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