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Table 2 Gene functions and mRNA primers used in this study. Primers were designed using EBV genes mapped to the B95.8 genome

From: Interferon-γ-inducible protein 16 (IFI16) is required for the maintenance of Epstein-Barr virus latency

EBV Gene

Temporal Class

Function

Forward primer (5′-3′)

Reverse primer (5′-3′)

BZLF1

Immediate early

transcriptional transactivator

ACGACGCACACGGAAACC

CTTGGCCCGGCATTTTCT

BALF2

Early

ssDNA binding protein

GTGAGCTACGCACCCGCCAT

CTGACCGGTTGACTTCG

BZLF2

Late

envelope glycoprotein gp42

CATCGCACTTGTTATTGTTC

CAGACCTCTACATTTGGTTTG

BcRF1

Late

vIL-10

GACAAAGGACGAGGTAGATAA

CTCCAGGTAGAATTGGATCATT

EBNA1

Latent

tethers EBV to sister chromatids in host cell

CCGCAGATGACCCAGGAGAA

TGGAAACCAGGGAGGCAAAT

IFI16

n/a

Host protein

CCCAAAGAAGATCATTGCCATAG

GTTTCGGTCAGCATTCACATC

β-Tubulin

n/a

Host protein

TCCAGATTGGCAATGCCTG

GGCCATCGGGCTGGAT

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Virology Journal

ISSN: 1743-422X