Fig. 6

IFI16 protein reduction is not dependent on late lytic transcripts. a Akata cells were left untreated (lane 1), incubated with 100 μg/mL PAA for 96 h (lane 2), with 30 ng/mL TPA for 96 h (lane 3), or with 100 μg/mL PAA for 1 h followed by the addition of 30 ng/mL TPA for an additional 96 h (lane 4), then immunoblotted for anti-IFI16 or anti-GAPDH. b MUTU-1 cells were treated the same as in A, except with 300 μg/mL of PAA and with 5 ng/mL of TGF-β in place of TPA. b & d Real time DNA PCR was performed to analyze relative EBV genome abundance in Akata (b) or MUTU-1 (d) cell lines. Primers specific to the latent EBNA1 gene were used and the level of DNA was normalized against the β-tubulin level. Results are presented as ± SD of data from three independent experiments and statistical analysis was done with a Student’s t test. ***, P < 0.001; ns, not significant