Fig. 3

Immunofluorescence analysis of the effect of lytic cycle induction by TPA on IFI16. BJAB (a) or Akata (b) cells were treated with 30 ng/mL of TPA or left untreated (uninduced) for 96 h. Cells were harvested, plated onto 10-well slides, fixed with ice-cold acetone, thoroughly washed, and blocked for 30 min at room temperature. Slides were stained with primary antibodies against the EBV glycoprotein gp350/220 (green) to visualize infected cells undergoing lytic replication and anti-IFI16 (red). Cell nuclei were visualized by DAPI staining (blue). Magnification, 40X. Red boxes are enlarged in the far right panels with white arrows indicating IFI16 puncta and yellow arrows showing gp350/220 puncta. A representative experiment out of three is shown