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Table 1 Investigation of feline leukemia virus (FeLV) for captive jaguarundis (Puma yagouaroundi) and putative infection outcomes

From: Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)

Animal

Zoo registry (CAD)

 

FeLV serological tests

FeLV molecular testsa

enFeLV

Putative infection outcome

 

Antibody

Antigen

Viral RNA

Proviral DNA

Indirect ELISA p45b

Indirect ELISA p15Ec

Western blottingb

Sandwich ELISAb

Snap testb

IHCd (Tissues)

Whole blood or buffy coat

Saliva

Tissues

Whole blood or buffy coatb

Saliva

Tissues

Whole blood or buffy coat

#1

20761

Nege

Neg

Neg

Posf

Pos

Pos

Pos

Pos

Pos

Pos

Pos

Pos

Neg

Progressive

#2

20762

Posg

Nth

Pos

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Abortive with seroconversion

#3

21566

Neg

Neg

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#4

21810

Neg

Neg

Neg

Pos

Neg

Pos

Pos

Nt

Nt

Pos

Nt

Nt

Neg

Progressive

#5

22096

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Nt

Neg

Neg

Nt

Neg

Neg

Non-infected

#6

23120

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#7

23851

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#8

23911

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#9

23956

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#10

24142

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#11

24189

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#12

24226

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#13

24227

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#14

24958

Neg

Neg

Neg

Neg

Neg

Neg

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#15

25244

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#16

25845

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#17

25846

Neg

Neg

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#18

26005

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#19

27088

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#20

27155

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#21

27156

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

#22

27689

Neg

Nt

Pos

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Abortive with seroconversion

#23

28018

Neg

Nt

Neg

Neg

Neg

Nt

Neg

Nt

Nt

Neg

Nt

Nt

Neg

Non-infected

Positive results/total tested

1/23

0/6

2/23

2/23

1/23

2/4

2/23

1/1

1/2

2/23

1/1

1/2

0/23

 
  1. aViral RNA and proviral DNA detection and quantification by RT-qPCR and qPCR, respectively [30]; enFeLV: investigation of endogenous proviral FeLV by qPCR [44]. bPreviously published test results [23]: Indirect ELISA – detection of serum or plasma antibodies against FeLV p45 [64]; Western blotting – detection of antibodies against FeLV proteins gp70, p58, p27, p15, and p12; blots are judged positive if they contain antibodies to at least three of these antigens [65]; sandwich ELISA – FeLV p27 antigen investigated in serum or plasma [66]; Snap test – FeLV p27 antigen measured in serum or fresh blood by Snap™ Combo FeLV Antigen/FIV Antibody Test Kit (IDEXX Laboratories, Inc., Westbrook, Maine 04092, USA); qPCR – proviral DNA detection in whole blood and buffy coat [30]. cIndirect ELISA – detection of plasma antibodies against the FeLV transmembrane protein p15E [32, 33]. Cutoff values were OD = 0.163. dIHC = FeLV antigen immunohistochemical analysis [35]. eNeg Negative test result. fPos Positive test result. gPos in indirect ELISA for detection of antibodies against FeLV p45 was 94% of the positive control used. hNt not tested
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Virology Journal

ISSN: 1743-422X