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Table 1 Investigation of feline leukemia virus (FeLV) for captive jaguarundis (Puma yagouaroundi) and putative infection outcomes

From: Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)

Animal Zoo registry (CAD)   FeLV serological tests FeLV molecular testsa enFeLV Putative infection outcome
  Antibody Antigen Viral RNA Proviral DNA
Indirect ELISA p45b Indirect ELISA p15Ec Western blottingb Sandwich ELISAb Snap testb IHCd (Tissues) Whole blood or buffy coat Saliva Tissues Whole blood or buffy coatb Saliva Tissues Whole blood or buffy coat
#1 20761 Nege Neg Neg Posf Pos Pos Pos Pos Pos Pos Pos Pos Neg Progressive
#2 20762 Posg Nth Pos Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Abortive with seroconversion
#3 21566 Neg Neg Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#4 21810 Neg Neg Neg Pos Neg Pos Pos Nt Nt Pos Nt Nt Neg Progressive
#5 22096 Neg Neg Neg Neg Neg Neg Neg Nt Neg Neg Nt Neg Neg Non-infected
#6 23120 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#7 23851 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#8 23911 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#9 23956 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#10 24142 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#11 24189 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#12 24226 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#13 24227 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#14 24958 Neg Neg Neg Neg Neg Neg Neg Nt Nt Neg Nt Nt Neg Non-infected
#15 25244 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#16 25845 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#17 25846 Neg Neg Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#18 26005 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#19 27088 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#20 27155 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#21 27156 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
#22 27689 Neg Nt Pos Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Abortive with seroconversion
#23 28018 Neg Nt Neg Neg Neg Nt Neg Nt Nt Neg Nt Nt Neg Non-infected
Positive results/total tested 1/23 0/6 2/23 2/23 1/23 2/4 2/23 1/1 1/2 2/23 1/1 1/2 0/23  
  1. aViral RNA and proviral DNA detection and quantification by RT-qPCR and qPCR, respectively [30]; enFeLV: investigation of endogenous proviral FeLV by qPCR [44]. bPreviously published test results [23]: Indirect ELISA – detection of serum or plasma antibodies against FeLV p45 [64]; Western blotting – detection of antibodies against FeLV proteins gp70, p58, p27, p15, and p12; blots are judged positive if they contain antibodies to at least three of these antigens [65]; sandwich ELISA – FeLV p27 antigen investigated in serum or plasma [66]; Snap test – FeLV p27 antigen measured in serum or fresh blood by Snap™ Combo FeLV Antigen/FIV Antibody Test Kit (IDEXX Laboratories, Inc., Westbrook, Maine 04092, USA); qPCR – proviral DNA detection in whole blood and buffy coat [30]. cIndirect ELISA – detection of plasma antibodies against the FeLV transmembrane protein p15E [32, 33]. Cutoff values were OD = 0.163. dIHC = FeLV antigen immunohistochemical analysis [35]. eNeg Negative test result. fPos Positive test result. gPos in indirect ELISA for detection of antibodies against FeLV p45 was 94% of the positive control used. hNt not tested
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