|
Animal
|
Zoo registry (CAD)
| |
FeLV serological tests
|
FeLV molecular testsa
|
enFeLV
|
Putative infection outcome
|
|---|
| |
Antibody
|
Antigen
|
Viral RNA
|
Proviral DNA
|
|---|
|
Indirect ELISA p45b
|
Indirect ELISA p15Ec
|
Western blottingb
|
Sandwich ELISAb
|
Snap testb
|
IHCd (Tissues)
|
Whole blood or buffy coat
|
Saliva
|
Tissues
|
Whole blood or buffy coatb
|
Saliva
|
Tissues
|
Whole blood or buffy coat
|
|---|
|
#1
|
20761
|
Nege
|
Neg
|
Neg
|
Posf
|
Pos
|
Pos
|
Pos
|
Pos
|
Pos
|
Pos
|
Pos
|
Pos
|
Neg
|
Progressive
|
|
#2
|
20762
|
Posg
|
Nth
|
Pos
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Abortive with seroconversion
|
|
#3
|
21566
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#4
|
21810
|
Neg
|
Neg
|
Neg
|
Pos
|
Neg
|
Pos
|
Pos
|
Nt
|
Nt
|
Pos
|
Nt
|
Nt
|
Neg
|
Progressive
|
|
#5
|
22096
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Neg
|
Nt
|
Neg
|
Neg
|
Non-infected
|
|
#6
|
23120
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#7
|
23851
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#8
|
23911
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#9
|
23956
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#10
|
24142
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#11
|
24189
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#12
|
24226
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#13
|
24227
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#14
|
24958
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#15
|
25244
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#16
|
25845
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#17
|
25846
|
Neg
|
Neg
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#18
|
26005
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#19
|
27088
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#20
|
27155
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#21
|
27156
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
#22
|
27689
|
Neg
|
Nt
|
Pos
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Abortive with seroconversion
|
|
#23
|
28018
|
Neg
|
Nt
|
Neg
|
Neg
|
Neg
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Nt
|
Nt
|
Neg
|
Non-infected
|
|
Positive results/total tested
|
1/23
|
0/6
|
2/23
|
2/23
|
1/23
|
2/4
|
2/23
|
1/1
|
1/2
|
2/23
|
1/1
|
1/2
|
0/23
| |
-
aViral RNA and proviral DNA detection and quantification by RT-qPCR and qPCR, respectively [30]; enFeLV: investigation of endogenous proviral FeLV by qPCR [44]. bPreviously published test results [23]: Indirect ELISA – detection of serum or plasma antibodies against FeLV p45 [64]; Western blotting – detection of antibodies against FeLV proteins gp70, p58, p27, p15, and p12; blots are judged positive if they contain antibodies to at least three of these antigens [65]; sandwich ELISA – FeLV p27 antigen investigated in serum or plasma [66]; Snap test – FeLV p27 antigen measured in serum or fresh blood by Snap™ Combo FeLV Antigen/FIV Antibody Test Kit (IDEXX Laboratories, Inc., Westbrook, Maine 04092, USA); qPCR – proviral DNA detection in whole blood and buffy coat [30]. cIndirect ELISA – detection of plasma antibodies against the FeLV transmembrane protein p15E [32, 33]. Cutoff values were OD = 0.163. dIHC = FeLV antigen immunohistochemical analysis [35]. eNeg Negative test result. fPos Positive test result. gPos in indirect ELISA for detection of antibodies against FeLV p45 was 94% of the positive control used. hNt not tested
