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Fig. 4 | Virology Journal

Fig. 4

From: An image cytometric technique is a concise method to detect adenoviruses and host cell proteins and to monitor the infection and cellular responses induced

Fig. 4

Image cytometric analysis of viral and cellular proteins. a NCI-H2452 cells were uninfected or infected with AdF35/Sur (103 or 104 vp/cell) for a period as indicated, stained with Ab against E1A and cleaved caspase-3, and analyzed with Tali image-based cytometer. Representative data are shown. The red lines are tentatively drawn to distinguish stained and unstained cells. A number in image data shows a percentage of the corresponding fraction. (−): uninfected. Bar graphs shows quantitative analysis of E1A single positive and E1A/cleaved caspase-3 double positive cells in (a) (104 vp/cell). Averages and SE bars are shown. b NCI-H2452 cells were infected as shown in (a) and stained with Ab against hexon and cleaved caspase-3. Bar graph shows quantitative analysis of hexon single positive and hexon/cleaved caspase-3 double positive cells in (b) (104 vp/cell). c MSTO-211H cells were uninfected or infected with AdF35/MK (103 or 104 vp/cell) for a period as indicated, stained with Ab against E1A and cleaved caspase-3, and analyzed with Tali image-based cytometer. Bar graph shows quantitative analysis of E1A single positive and E1A/cleaved caspase-3 double positive cells in (c) (104 vp/cell)

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Virology Journal

ISSN: 1743-422X