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Fig. 3 | Virology Journal

Fig. 3

From: An image cytometric technique is a concise method to detect adenoviruses and host cell proteins and to monitor the infection and cellular responses induced

Fig. 3

Image cytometric analysis of hexon and cleaved caspase-3. a MSTO-211H cells were uninfected or infected with AdF35/Sur (103 or 104 vp/cell) for a period as indicated, stained with Ab against hexon and cleaved caspase-3, and analyzed with Tali image-based cytometer. Representative data are shown. The red lines are tentatively drawn to distinguish stained and unstained cells. A number in image data shows a percentage of the corresponding fraction. b Quantitative analysis of hexon single positive and hexon/cleaved caspase-3 double positive cells (104 vp/cell). Averages and SE bars are shown. c Hexon intensity per cell analyzed with image cytometry. The hexon expression level per cell, corresponding each dot in (a), was plotted and the cell numbers are shown in the abscissa axis. Cells showing less than 1800 (arbitrary unit) at the hexon fluorescent intensity were gated out. A red line shows hexon intensity at 2000 and the hexon intensity of positively stained cells (>2000 units) was calculated. An average unit of the intensity of the positive cells and SE are shown with statistical analysis (ANOVA)

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