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Fig. 2 | Virology Journal

Fig. 2

From: An image cytometric technique is a concise method to detect adenoviruses and host cell proteins and to monitor the infection and cellular responses induced

Fig. 2

Image cytometric analysis of E1A and cleaved caspase-3. a MSTO-211H cells were uninfected or infected with AdF35/Sur (103 or 104 vp/cell) for a period as indicated, stained with Ab against E1A and cleaved caspase-3, and analyzed with Tali image-based cytometer. Representative data are shown. The red lines are tentatively drawn to distinguish stained and unstained cells. A number in image data shows a percentage of the corresponding fraction. b Quantitative analysis of E1A single positive and E1A/cleaved caspase-3 double positive cells (104 vp/cell). Averages and SE bars are shown. c Western blot analysis to detect E1A expression in MSTO-211H cells uninfected or infected with AdF35/Sur at a vp/cell dose as indicated for 24 h. (−): uninfected. d E1A intensity per cell analyzed with image cytometry. The E1A expression per cell, corresponding each dot in (a), was plotted and the dot numbers are shown in the abscissa axis. Cells showing less than 1800 (arbitrary unit) at the E1A fluorescent intensity were gated out. A red line shows E1A intensity at 2000 and the E1A intensity of positively stained cells (>2000 units) was calculated. An average unit of the intensity of the positive cells and SE are shown with statistical analysis (ANOVA)

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