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Fig. 1 | Virology Journal

Fig. 1

From: An image cytometric technique is a concise method to detect adenoviruses and host cell proteins and to monitor the infection and cellular responses induced

Fig. 1

AdF35/Sur-mediated cytotoxicity. a MSTO-211H cells were uninfected or infected with AdF35/Sur at a vp/cell dose as indicated, and the numbers of adherent and floating cells were counted. Average and SE bars are shown. (−): uninfected. b MSTO-211H cells were uninfected or infected with AdF35/LacZ or AdF35/Sur (104 vp/cell) and live cell numbers were counted with a trypan blue dye exclusion assay. c MSTO-211H cells were uninfected or infected with AdF35/Sur at a vp/cell as indicated for 1 day, and were stained with anti-E1A Ab and propidium iodide. d MSTO-211H cells were uninfected or infected with AdF35/Sur (104 vp/cell) as indicated and were examined for the expression levels of viral and cellular proteins with Western blot analysis. E1A and hexon showed multiple bands. Tubulin-α was used as a loading control

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