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Table 1 Results of preliminary immunoreactivity studies and isotyping of the finally selected monoclonal antibodies

From: Production of highly and broad-range specific monoclonal antibodies against hemagglutinin of H5-subtype avian influenza viruses and their differentiation by mass spectrometry

  1. The affinity-purified monoclonal antibodies (mAbs) were tested using ELISAs that targeted the recombinant proteins based on the ectodomain (rHA) or HA1 subunit (rHA1) of the H5 hemagglutinins (HAs) and the avian influenza viruses (AIVs) of H1-H16 subtypes as antigens. The HA antigens are listed in Additional file 1: Tables S2 and S4. The characteristics of the rHA and rHA1 proteins are presented in Additional file 1: Figures S1-S6 and Table S3. On this basis, the proteins were classified as conformational (properly folded) and non-conformational (misfolded) antigens. The amino acid sequence identities for the HA1 subunits of the H5 HAs were obtained using the BLAST program on NCBI by alignments against the 17–338-aa sequence of HA from the A/Bar-headed Goose/Qinghai/12/05(H5N1) HPAIV. The sequence homology with the non-H5 HAs was not determined (n.d.). The immunoreactivity studies and isotyping were performed in the presence of control samples as described in the Methods. The mean absorbance values for blank control samples were subtracted. Positivity and negativity in the tests with the specified antigens are indicated by plus and minus symbols, respectively. The underlying raw data are included in Additional file 3: Tables S8, S9 and S10