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Table 1 Real-time PCR Primers and probes specifications

From: Association of IFITM3 rs12252 polymorphisms, BMI, diabetes, and hypercholesterolemia with mild flu in an Iranian population

Primers/Probes

Sequence (5′ > 3′)

Working Concentration

InfA Forward

GAC CRA TCC TGT CAC CTC TGA C

40 μM

InfA Reverse

GCA TTY TGG ACA AAK CGT CTA

40 μM

InfA Probea

TGC AGT CCT CGC TCA CTG GGC ACG

10 μM

SW InfA Forward

GCA CGG TCA GCA CTT ATY CTR AG

40 μM

SW InfA Reverse

GTG RGC TGG GTT TTC ATT TGG TC

40 μM

SW InfA Probeb

CYA CTG CAA GCC CA“T” ACA CAC AAG CAG GCA

10 μM

Flu B Primera M

GAGACACAATTGCCTACCTGCTT

10 pm

Flu B Primerb M

TTCTTTCCCACCGAACCAAC

10 pm

B Probe M

AGAAGATGGAGAAGGCAAAGCAGAACTAGC

5 pm

Rnase P Forward

AGATTTGGACCTGCGAGCG

40 μM

Rnase P Reverse

GAGCGGCTGTCTCCACAAGT

40 μM

Rnase P Probea

TTCTGACCTGAAGGCTCTGCGCG

10 μM

  1. aTaqMan probes are labeled at 5′- end with the reporter molecule 6- carboxyfluorescein (FAM) and with the quencher Blackhole Quencher 1 (BHQ1) at the 3′- end
  2. bTaqMan probes are labeled at 5′- end with the reporter molecule 6- carboxyfluorescein (FAM) and quenched internally at a modified “T” residue with BHQ1 with a modified 3′- end to prevent probe extension by Taq polymerase