Table 1 PCR primers used in this study
Primer | Primer sequences (5′ → 3′) |
|---|---|
DHAV-Seq-1F | TTT GAA AGC GGG TGC AT |
DHAV-Seq-1R | TTA TAG TGT GTG GGA CTC GAC C |
DHAV-Seq-2F | GAC TAG TTC CTG AGG GAC AGA TGT T |
DHAV-Seq-2R | TCC CTG ATT GTC AAA TGG TCG G |
DHAV-Seq-3F | ACA ACT GGT GGT GCC ATT TGT GT |
DHAV-Seq-3R | CTG CCA AAA GTT GCC TCT GAT GTG C |
DHAV-Seq-4F | TGG ATG ACC TCA CTT CAG AGT ATG C |
DHAV-Seq-4R | TTG ACT GCA TGT GAT CAC CTG CTG G |
DHAV-Seq-5F | TAA ATG GTG AAG TCA CAA TCA AG |
DHAV-Seq-5R | GGC CAA AAT CAT CAA AAG CAT |
DHAV-Seq-6F | CTC TTG GTA TAT GGA TAT CTG GTG GT |
DHAV-Seq-6R | GTA GCA ATC AAT TTA GAC ACA TCT |
DHAV-Seq-7F | AGA TGA GAT TAG GGA CAT |
DHAV-Seq-7R | TGG GTA TAA CAT CAC TAC TC |
DHAV-Seq-8F | AGA CAC ATG TTG CTG AAA AAC T |
DHAV-Seq-8R | AGA ACA CAG TCA TCC CCA TAA CT |
DHAV-Seq-9F | CTG ATG AGA TAT GGC AGG TA |
DHAV-Seq-9R | TTT TTT TTT AGG TAG GGT AGG GAA T |
pIR-BamHI-XhoI-F | GGG ATC CTA CTC TAG ACA TAA TCA GCC ATA CCA CAT TTG TAG AGG |
pIR-BamHI-AscI-R | GAA GGA TCC ACT GGC GCG CCT CGA GAT CTG AGT CCG GTA G |
5HeadRibo-F | ACA TGG CGC GCC ACA TCA TCT GAT GAG TCC GTG AGG ACG AAA CGG TAC CCG CGT GAG GAC GAA ACG GTA CCC GGT ACC GTC ATT TTG AAA GCG GGT GCA TGC ATG GCC AT |
5HeadRibo-R | AGG TGG ATC CAC TAT TGT CAC CTT C |
3endRibo-F | CTG ATG AGA TAT GGC AGG TA |
3endRibo-R | GCT CTA GAG TCC CAT TCG CCA TTA CCG AGG GGA CGG TCC CCT CGG AAT GTT GCC CAG CCG GCG CCA GCG AGG AGG CTG GGA CCA TGC CGG CCT TTT TTT TTT TTT TTT TTT TTA GGT AGG GTA GGG AAT AGT AAA GT |
Cloning 2-F | TAG TGG ATC CAC CTG AAA CAC C |
Cloning 2-R | ACC AGAT ATC CAT ATA CCA AGA GGT T |
BamH I-detect-F | ACA ACT GGT GGT GCC ATT TGT GT |
BamH I-detect-R | TTG ACT GCA TGT GAT CAC CTG CTG G |
RT-qPCR-F | AGA CAC ATG TTG CTG AAA AAC T |
RT-qPCR-R | AGA ACC AGT TGT CGT TTG GTC |
DHAV-1-Probe | Cy5-ATG CCA TGA CAC TAT CTC ATA TGA GTC AGC-BHQ-2 |