Fig. 4

Isoform changes in the ZIKV infected hNPCs transcriptome. a A gene with isoform changes of at least 2-fold (FPKM ≥ 1, FDR ≤ 0.05) was plotted. A Total of 691 significant isoform changes were found with 355 gene isoforms showing increase of expression and 336 showing reduction. X-axis shows uninfected hNPCs control, and Y-axis shows the amount of isoform expression after infection. Red dots indicate up-regulated gene isoforms and blue dots show reduced isoform expression; b. the overlapping genes of AS and ISO; c, d. GO analysis and pathway analysis of down-regulated isoforms, p value ≤ 0.05; e, f. GO analysis and pathway analysis of up-regulated isoforms, p value ≤ 0.05. g,h. expression level of E2F1, E2F2, CXB4, HEY1, TNFRSF10D, and BBC3. Each group consists of two biological replicates. *: FDR < 0.05, **:FDR 0.01. The x-axis of c/d/e/f graphs is the –log ₁₀ ^{p value}