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Table 3 An overview of accuracy testing by three methods

From: Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood

Set

Method

Assay (3 LC runs per assay)

Reference Values

A

Negative blood spiked with MPX virus

1, 2, 3

Converted pfu to GC/ml

B

Negative blood spiked with extracted DNA

1, 2, 3

Used GC values from Set A

C

Negative blood spiked with extracted DNA

1, 2, 3

Used GC values from Set C

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Virology Journal

ISSN: 1743-422X