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Table 1 Acceptance criteria for standards, controls, and test samples

From: Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood

Standards & Controls

Acceptable Range (GC/5 μl)Low to High

Acceptable %CV

PEC

1.58 × 103

1.58 × 104

≤ 40%

50 HA Standard

1.58 × 101

1.58 × 102

≤ 40%

500 HA Standard

1.58 × 102

1.58 × 103

≤ 30%

5000 HA Standard

1.58 × 103

1.58 × 104

≤ 30%

50,000 HA Standard

1.58 × 104

1.58 × 105

≤ 30%

500,000 HA Standard

1.58 × 105

1.58 × 106

≤ 30%

5,000,000 HA Standard

1.58 × 106

1.58 × 107

≤ 30%

PTS

NA

NA

≤ 30%

Efficiency

1.8

2.2

< 10%

  1. PEC positive extraction control
  2. PTS positive test sample
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Virology Journal

ISSN: 1743-422X