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Table 1 Acceptance criteria for standards, controls, and test samples

From: Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood

Standards & Controls Acceptable Range (GC/5 μl)Low to High Acceptable %CV
PEC 1.58 × 103 1.58 × 104 ≤ 40%
50 HA Standard 1.58 × 101 1.58 × 102 ≤ 40%
500 HA Standard 1.58 × 102 1.58 × 103 ≤ 30%
5000 HA Standard 1.58 × 103 1.58 × 104 ≤ 30%
50,000 HA Standard 1.58 × 104 1.58 × 105 ≤ 30%
500,000 HA Standard 1.58 × 105 1.58 × 106 ≤ 30%
5,000,000 HA Standard 1.58 × 106 1.58 × 107 ≤ 30%
PTS NA NA ≤ 30%
Efficiency 1.8 2.2 < 10%
  1. PEC positive extraction control
  2. PTS positive test sample