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Fig. 3 | Virology Journal

Fig. 3

From: HPV11 E6 mutation by overexpression of APOBEC3A and effects of interferon-ω on APOBEC3s and HPV11 E6 expression in HPV11.HaCaT cells

Fig. 3

Mutation and the mRNA expression of E6 gene in the recircularized HPV11 DNA, HPV11.HaCaT and A3A-HPV11.HaCaT cells. E6 DNA amplified at a denaturation temperature of 86 °C was excised, cloned, and sequenced. Results are shown as mutation matrices (a-d) and alignmants (e-g). The number of C-to-T and G-to-A conversions in each sequence is indicated (a-d). nt, nucleotides; P3, passage 3; P8, passage 8. Mutated positions of HPV11 E6 gene amplified from P8-HPV11.HaCaT (e) and A3A-HPV11.HaCaT (f and g). qRT-PCR analysis was performed to determine relative E6 mRNA expression in HPV11.HaCaT and A3A-HPV11.HaCaT cells (h). Data was expressed as means ± SD from three independent experiments and presented as fold increase in E6 mRNA expression relative to HPV11.HaCaT cells. *p < 0.05 vs HPV11.HaCaT using Student t-test

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