Skip to main content

Advertisement

Table 2 Summary of BLV genotyping methods

From: Epidemiology and genetic diversity of bovine leukemia virus

Genotyping method Amplified BLV region Amplicon size (bp) Enzymes Phylogenetic approaches Classification result Reference
PCR-RFLP Partial env-gp51 region 444 BamHI, BglI, HaeIII, BclI, PvuII, DraI, HindIII, HpaII, StuI, TaqI   7 groups: A, B, C, D, E, F, G Fechner et al., 1997 [90]
Licursi et al., 2002 [91]
Asfaw et al., 2005 [95]
RFLP + sequencing Partial gp51 sequencing 400–444 BamHI, BclI, PvuII, GmbH NJ; MP; ML RFLP-based type: Australian type, Argentine type, Belgium type, Japanese type; Sequence-based type: Argentine cluster, European cluster, Japan and German isolate cluster; groups I–IV; or genotypes 1–8 Monti et al., 2005 [49]
Felmer et al., 2005 [93]
Camargos et al., 2007 [122]
PCR-sequencing Partial gp51 sequencing 346–444   NJ; ML; BI Japanese group, Argentine group, European group; or genotypes 1–8 Camargos et al., 2002 [121]
Licursi et al., 2003 [92]
Matsumura et al., 2011 [98]
Rola-Luszczak et al., 2013 [99]
Polat et al., 2015 [74]
Ochirkhuu et al., 2016 [77]
Polat et al., 2016 [75, 76]
  Sequencing of partial or full gp51 gene sequences 444–903   NJ; ML; BI Up to 10 BLV genotypes Moratorio et al., 2010 [126]
Balic et al., 2012 [97]
Lee et al., 2015 [100]
Lee et al., 2016 [101]
  Sequencing of env (full gp51 and/or gp30 genes) up to 1548   NJ; ML; BI Consensus cluster, US Californian cluster, European cluster, Costa Rican cluster; or genotypes 1–10 Zhao et al., 2007 [109]
Rodriguez et al., 2009 [96]
Yang et al., 2016 [131]
Full BLV genome sequencing BLV complete genome 8714   ML genotypes −1, −2, −4, −6, −9, and −10 Polat et al., 2016 [75, 76]
  1. BI Bayesian inference, BLV bovine leukemia virus, NJ neighbor-joining, ML maximum-likelihood, MP maximum-parsimony, RFLP restriction fragment length polymorphism