Fig. 5

ALT and mRNA levels of ISGs and immune factors after HI with different concentrations of HBV plasmid DNA. 1 μg, 5 μg, 10 μg or 100 μg pAAV-HBV1.2 plasmid DNA were injected into BALB/c mice, respectively. The mice sera were collected at 1, 4, 10 dpi and the total mRNA was extracted form liver tissue of the mice at 3 dpi. a Serum ALT levels were measured on full automated biochemistry analyzer and the mRNA expression levels of b IRF3, ISG15, OAS, PKR and c IFNγ, TNFα, TGFβ, IL-6, IL-10 and PDL1 were determined by qRT-PCR. β-actin was used for normalization. Each sample was run in duplicate and at least four mice per group were analyzed. Differences between the groups were analyzed by using the One-way ANOVA. Statistically significant differences between the pAAV-HBV1.2 plasmid DNA injected groups and the PBS injected control group were indicated by * for p < 0.05, ** for p < 0.01 and *** for p < 0.001