Fig. 3From: Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in miceWestern blot analysis of GP and VP40 protein expression in the purified MARV VLPs. 10 μg of the purified MARV VLPs were mixed with reducing (with β-mercaptoethanol) protein sample buffer, heated at 95 °C for 5 min, and then subjected to 10% SDS-PAGE with different gels. Two different gels were transferred onto a polyvinylidene fluoride (PVDF) membrane for the Western blot analysis, respectively. a GP was incubated with mouse anti-MARV GP polyclonal antibody (purified MARV VLPs, lane 1; control, lane 2). b VP40 proteins was incubated with mouse anti-MARV VP40 polyclonal antibody (purified MARV VLPs, lane 1; control, lane 2)Back to article page