Fig. 5

Detection of the effect of Viperin expression on CSFV binding and entry. a. Virus binding assay. Cells were inoculated with CSFV (MOI = 1) for 1 h on ice, washed with PBS. Cell-bound CSFV was measured by qRT-PCR. b. Entry assay. Virus inoculum was removed after 1 h of binding on ice, and then cells were washed with PBS and incubated in culture medium for 2 h at 37 °C. Cells were washed and trypsinated to remove bound but not entered virus before qRT-PCR detection. The relative genome levels of PK-Vi cells were normalized to those of PK-C1 cells (referred as 1). Data was shown as the mean ± S.D. from three independent experiments