Skip to main content
Fig. 4 | Virology Journal

Fig. 4

From: Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro

Fig. 4

Over-expression of Viperin inhibits CSFV replication in PK-Vi cell line. a and b. CSFV genome RNA expression (a) and progeny virus (b) in PK-Vi was determined by qRT-PCR and virus titration. At 12, 24, 48 and 72 hpi, cell cultures were collected and lysed by three freeze and thaw cycles, then subjected to corresponding tests. c. Level of CSFV protein Npro was determined by Western blot analysis. Cell lysates were harvested at 24, 48 and 72 hpi and detected with anti-Npro antibody. An anti-β-actin antibody was used as an internal loading control. Protein spots levels were determined using ImageJ quantification software. Relative Npro levels at each time point was that in PK-Vi group to that of PK-C1 group (referred as 1)

Back to article page
\