Fig. 3
From: Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro
Generation and validation of stably-transfected cell lines. a and b. Western blot identification of PK-Vi and PK-C1 by anti-GFP antibody (a) and anti-Viperin antibody (b). lane 1, PK-C1; lane 2, PK-Vi. c. PK-Vi, PK-C1 and PK-15 cells were seeded into 96-well plate and incubated for 5 days and cell viability was determined by MTT assay. The cell viability of PK-15 was used as control (100%). Data are expressed as mean (% of control) ± S.D. d. Confocal microscopy examination of PK-Vi and PK-C1. Nuclei were stained with DAPI (Beyotime, Biotech, China) and the cells were examined by confocal laser scanning microscopy (PE, Ultra View VOX). Scale bar indicated 20 μm