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Fig. 1 | Virology Journal

Fig. 1

From: Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro

Fig. 1

Detection of Viperin expression in 3D4/21 cells and PBMCs. a and b. 3D4/21 cells (a) and PBMCs (b) were stimulated with: 1) NDV and CSFV (MOI = 1); 2) NDV alone; 3) CSFV alone; 4) medium (negative control). c and d. 3D4/21 cells (c) and PBMCs (d) were stimulated with: 1) PRV and CSFV (MOI = 1); 2) PRV alone; 3) CSFV alone; 4) medium (negative control). Levels of Viperin mRNA presented in cell cultures of 0, 6, 12, 24 and 48 hpi were determined by relative qRT-PCR. Relative quantification of Viperin mRNA was that in treated group to that of untreated control group. The relative fold change of Viperin level was expressed as –ΔΔCt. Each treatment was performed in triplicate and data was shown as the mean ± S.D. Columns at each time point marked with * (P < 0.05) or ** (P < 0.01) are significantly different from each other

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