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Table 1 Probe sequences for each strain and their thermodynamic parameters calculated. Equilibrium constants and ratio of probe predicted to fluoresce for each probe

From: Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes

Probe sequence Kstemloop KdetectionT (μM−1) KcaptureT (μM−1) Fraction of fluorophore predicted to be fluorescing with Target Fraction of fluorophore predicted to be fluorescing with Non-Target
Clone13:
FAM-cgtaagTTCCTCTCACGaacttcg-BHQ-PEG9-acattcacctggactttgtcagactc
62.35 82.53 1.43 × 108 0.5447 0.0053
Armstrong:
FAM-cgtagtGATTCTTCACtacg-BHQ-PEG9-agcgggcacattcacctgg
26.44 33.93 6.9 × 109 0.3188 0.0276
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