Table 1 Probe sequences for each strain and their thermodynamic parameters calculated. Equilibrium constants and ratio of probe predicted to fluoresce for each probe
From: Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes
Probe sequence | Kstemloop | KdetectionT (μM−1) | KcaptureT (μM−1) | Fraction of fluorophore predicted to be fluorescing with Target | Fraction of fluorophore predicted to be fluorescing with Non-Target |
|---|---|---|---|---|---|
Clone13: FAM-cgtaagTTCCTCTCACGaacttcg-BHQ-PEG9-acattcacctggactttgtcagactc | 62.35 | 82.53 | 1.43 × 108 | 0.5447 | 0.0053 |
Armstrong: FAM-cgtagtGATTCTTCACtacg-BHQ-PEG9-agcgggcacattcacctgg | 26.44 | 33.93 | 6.9 × 109 | 0.3188 | 0.0276 |