Fig. 3From: Identification of a novel linear B-cell epitope using a monoclonal antibody against the carboxy terminus of the canine distemper virus nucleoprotein and sequence analysis of the identified epitope in different CDV isolatesIdentification of minimal linear epitope recognized by mAb C8. a C8 was screened by indirect ELISA against a panel of 23 overlapping peptides derived from the CDV nucleoprotein protein (aa 401–523). The SP2/0 cell culture supernatant was used as the negative antibody control. The error bars display the standard deviation of three experimental repeats. b Mab C8 was screened against a series of truncated peptides, with amino acid residues progressively deleted from the amino or carboxyl terminus, to determine the minimal amino acid sequences required for MAb binding. The SP2/0 cell culture supernatant was used as the negative antibody control. c The name and sequence of the peptides used are included in the tableBack to article page