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Fig. 3 | Virology Journal

Fig. 3

From: Identification of a novel linear B-cell epitope using a monoclonal antibody against the carboxy terminus of the canine distemper virus nucleoprotein and sequence analysis of the identified epitope in different CDV isolates

Fig. 3

Identification of minimal linear epitope recognized by mAb C8. a C8 was screened by indirect ELISA against a panel of 23 overlapping peptides derived from the CDV nucleoprotein protein (aa 401–523). The SP2/0 cell culture supernatant was used as the negative antibody control. The error bars display the standard deviation of three experimental repeats. b Mab C8 was screened against a series of truncated peptides, with amino acid residues progressively deleted from the amino or carboxyl terminus, to determine the minimal amino acid sequences required for MAb binding. The SP2/0 cell culture supernatant was used as the negative antibody control. c The name and sequence of the peptides used are included in the table

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