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Fig. 1 | Virology Journal

Fig. 1

From: Identification of a novel linear B-cell epitope using a monoclonal antibody against the carboxy terminus of the canine distemper virus nucleoprotein and sequence analysis of the identified epitope in different CDV isolates

Fig. 1

Expression and purification of recombinant CDV N protein (C-terminal 401-523aa). a SDS-PAGE analysis of the recombinant N protein (C-terminal 401-523aa).Lane M, molecular weight marker; Lane 1, proteins expressed in E. coli without IPTG induction. Lane 2, supernatant of E. coli cell lysates expressed with IPTG induction; Lane 3, inclusion bodies of E. coli cell expressed with IPTG. b SDS-PAGE analysis of the purified N protein (C-terminal 401-523aa). lane 1–8, purified recombinant protein with different iminazole concentrations: 20 mM, 40 mM, 60 mM, 80 mM, 120 mM, 160 mM, 180 mM, and 200 mM. c Western blot analysis of purified protein.Lane M, molecular weight marker; Lane 1, the binding of purified recombinant protein with CDV-positive dog sera

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