Fig. 3

Evaluation of serotype specificity of anti-E mAbs. a Dot blot analysis using lysates from C6/36 cells infected with clinical DV strains collected in Taiwan as a target, which were spotted on NC membrane and reacted with DV1-E1 and DV1-E2, respectively. b Identification of the serotype specificity of anti-E mAbs by IFA. Prototype or clinical samples collected in Taiwan of DV-infected C6/36 cells were scraped and fixed on slides, then reacted with DV1-E1 and DV1-E2. A monoclonal antibody (HB114) directed against dengue E protein was reacted with the virus-infected cells as a positive control. JEV (NAK strain) or mock-infected C6/36 cells were stained with the anti-E mAbs as negative controls