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Fig. 6 | Virology Journal

Fig. 6

From: Viral metagenomics of aphids present in bean and maize plots on mixed-use farms in Kenya reveals the presence of three dicistroviruses including a novel Big Sioux River virus-like dicistrovirus

Fig. 6

Deduced genome organization of the (BSRV)-like virus. The positive-sense single stranded RNA genome of the BSRV-like virus is approximately 10.2Kb and 3′-polyadenylated. The 5′-untranslated region (5′-UTR) and intergenic region (IGR) are 680 and 594 nucleotides (nt) long, respectively, and are internal ribosome entry sites (IRESs) for translation of open reading frames (ORFs) 1 and 2 (gray boxes). A VPg molecule is likely attached to the 5’end of the genomic RNA. The IGR-IRES for this virus is longer than that of other dicistroviruses. By comparison, RhPV is the only other dicistrovirus with a long IGR-IRES (533 nt), while the ALPV IGR-IRES is 197 nt long (see Fig. 2). ORF1 (6024 nt) is predicted to encode a non-structural protein precursor of 2008 amino acid residues. ORF2 is 2262 nucleotides long and is translated into a 754-amino acid long structural protein precursor and if consistent with other dicistroviruses, self-cleaves into mature capsid proteins. Locations of putative helicase and RdRp domains encoded by ORF1 and three putative structural proteins encoded by ORF2 (shaded blue) were obtained by searching for homology in the NCBI conserved domain database. As in Fig. 2, only those putative functional domains in the encoded proteins that can be deduced with a high degree of certainty are shown

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