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Fig. 3 | Virology Journal

Fig. 3

From: Viral metagenomics of aphids present in bean and maize plots on mixed-use farms in Kenya reveals the presence of three dicistroviruses including a novel Big Sioux River virus-like dicistrovirus

Fig. 3

Phylogenetic analyses and pairwise sequence similarity comparisons of Aphid lethal paralysis virus (ALPV). a ALPV isolates identified from aphids in Kenya (KE) this study (KE Aphid P7 and KE Aphid P9) and an isolate from this study found in maize plants in Kenya (KE Maize) were analysed alongside six other ALPV genomic sequences available at GenBank (from China, South Africa, Israel, East Timor USA and Spain). Analysis revealed two main clades. The isolates KE Aphid P7 and KE Aphid P9 clustered in a subclade together with the ALPV isolate from Israel in the larger clade and KE Maize clustered with isolates from China, East Timor and South Africa (orange dashed box). Drosophila C virus (DCV) was used as the out-group. b The arrangement of the sequences in the pairwise comparison is based on a neighbour-joining tree from the aligned sequences. Pairwise alignments revealed similarities as low as 82% when isolates in different clades are compared. In both analyses, the sequences were aligned using MUSCLE. The phylogenetic tree was constructed using the Neighbor-Joining method with 1000 bootstraps in MEGA6. Asterisks indicate ALPV isolates identified in this study

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Virology Journal

ISSN: 1743-422X