Table 3 Compared detection times of different lyssavirus positive and negative field samples (sample subset II [27]) by measured by R14 RT-qPCR, HighSpeed RT-qPCR and RT-RPA. Cq valued from RT-qPCRs were converted into detection times in minutes (min) to compare them to the detection time of RT-RPA. Numbers are rounded mean values and in brackets given are mean Cq values
From: Development of molecular confirmation tools for swift and easy rabies diagnostics
Lab ID | Virus species | Species | Origin | Lineage | Detection by Standard R14 RT-qPCR min (Cq) | Detection by HighSpeed RT-qPCR min (Cq) | Detection by RT-RPA min |
|---|---|---|---|---|---|---|---|
34,202 | RABV | Dog | Yugoslavia | Cosmopolitan | 45.0 (15.75) | 12.0 (13.80) | 5.0 |
13,491 | RABV | Dog | Ethiopia | Cosmopolitan | 47.5 (18.03) | 13.5 (15.59) | 5.5 |
34,203 | RABV | Wolf | Yugoslavia | Cosmopolitan | 47.5 (17.90) | 14.0 (16.51) | 5.0 |
13,099 | RABV | Dog | Taiwan | South-East Asia | 48.0 (18.53) | 14.0 (16.16) | 5.0 |
13,255 | RABV | Human | Chile | Cosmopolitan | 40.5 (12.37) | 10.0 (11.01) | 5.0 |
8192 | RABV | Fox | Bosnia-Herzegovina | Cosmopolitan | 51.0 (20.87) | 14.5 (17.38) | 5.0 |
3139 | RABV | Fox | Germany | Cosmopolitan | 48.0 (18.40) | 14.0 (16.16) | 5.0 |
13,133 | RABV | Cat | Nigeria | Cosmopolitan | 50.5 (20.46) | 14.5 (17.22) | 5.0 |
13,242 | RABV | Bat | South America | American bat variant | 52.5 (21.91) | 13.0 (15.01) | 5.0 |
13,209 | RABV | Mongoose | South America | Cosmopolitan | 46.0 (16.63) | 12.5 (14.08) | 5.0 |
13,206 | RABV | Raccoon | North America | raccoon variant | 61.5 (29.16) | 24.0 (31.83) | negative |
13,200 | RABV | Skunk | USA | skunk variant | 48.0 (18.38) | 14.0 (15.98) | 6.0 |
4131 | RABV | Fox | Czech Republic | Cosmopolitan | 49.5 (19.48) | 15.5 (18.58) | 4.5 |
13,117 | RABV | Dog | Algeria | Cosmopolitan | 44.0 (15.21) | 11.5 (12.59) | 5.0 |
4134 | RABV | Fox | Czech Republic | Cosmopolitan | 50.0 (19.74) | 16.5 (20.29) | 4.5 |
13,056 | RABV | Dog | Turkey | Middle East | 48.5 (18.68) | 13.5 (18.94) | 6.0 |
13,112 | RABV | Human | Malaysia | South-East Asia | 46.0 (16.85) | 14.0 (16.54) | 5.0 |
13,208 | RABV | Vampire bat | South America | American bat variant | 51.5 (21.31) | 18.25 (22.94) | 9.0 |
13,015 | RABV | Arctic fox | Norway | Arctic | 49.5 (19.73) | 13.75 (16.14) | 4.5 |
13,017 | RABV | Arctic fox | Norway | Arctic | 53.0 (22.50) | 15.5 (18.81) | 6.0 |
16,854 | RABV | Fox | Kosovo | Cosmopolitan | 49.0 (19.01) | 13.0 (14.94) | 5.5 |
13,512 | RABV | – | South Africa | Cosmopolitan | 43.5 (14.89) | 10.75 (11.57) | 4.5 |
13,114 | RABV | Human | Malaysia | South-East Asia | 47.5 (17.90) | 12.0 (13.34) | 5.0 |
13,093 | RABV | Camel | Emirates | Cosmopolitan | 48.5 (18.83) | 14.5 (17.45) | 8.0 |
20,299 | RABV | Cattle | Iraq | Cosmopolitan | 47.0 (17.56) | 16.0 (19.52) | 5.0 |
20,299 1:2 | RABV | Cattle | Iraq | Cosmopolitan | 45.5 (19.20) | 12.5 (14.07) | 5.0 |
20,299 1:4 | RABV | Cattle | Iraq | Cosmopolitan | 50.0 (19.84) | 12.0 (13.10) | 6.0 |
20,299 1:8 | RABV | Cattle | Iraq | Cosmopolitan | 50.0 (20.04) | 13.0 (15.14) | 5.0 |
20,299 1:16 | RABV | Cattle | Iraq | Cosmopolitan | 51.0 (20.74) | 13.5 (15.85) | 6.0 |
20,299 1:32 | RABV | Cattle | Iraq | Cosmopolitan | 52.0 (21.71) | 14.0 (16.08) | 6.0 |
20,299 1:64 | RABV | Cattle | Iraq | Cosmopolitan | 53.0 (22.60) | 14.5 (17.15) | 6.0 |
20,299 1:128 | RABV | Cattle | Iraq | Cosmopolitan | 55.0 (24.22) | 15.0 (18.22) | 6.0 |
2498 | RABV | Cat | Germany | Cosmopolitan | 71.5 (37.19) | 23.5 (30.71) | 11.0 |
10,280 | EBLV-1 | Sheep | experimental | – | negative | negative | negative |
10,270 | EBLV-2 | Sheep | experimental | – | negative | negative | negative |
34,494 | BBLV | Bat | Germany | – | negative | negative | negative |
34,495 | BBLV | Bat | Germany | – | negative | negative | negative |
12,861 | DUVV | Human | South Africa | – | 69.0 (35.05) | 14.5 (17.40) | 5.5 |
33,341 | – | Wolf | Germany | – | negative | negative | negative |
33,342 | – | Wolf | Germany | – | negative | negative | negative |
33,343 | – | Wolf | Germany | – | negative | negative | negative |
33,344 | – | Wolf | Germany | – | negative | negative | negative |
33,345 | – | Wolf | Germany | – | negative | negative | negative |