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Table 3 Compared detection times of different lyssavirus positive and negative field samples (sample subset II [27]) by measured by R14 RT-qPCR, HighSpeed RT-qPCR and RT-RPA. Cq valued from RT-qPCRs were converted into detection times in minutes (min) to compare them to the detection time of RT-RPA. Numbers are rounded mean values and in brackets given are mean Cq values

From: Development of molecular confirmation tools for swift and easy rabies diagnostics

Lab ID Virus species Species Origin Lineage Detection by Standard R14 RT-qPCR min (Cq) Detection by HighSpeed RT-qPCR min (Cq) Detection by RT-RPA min
34,202 RABV Dog Yugoslavia Cosmopolitan 45.0 (15.75) 12.0 (13.80) 5.0
13,491 RABV Dog Ethiopia Cosmopolitan 47.5 (18.03) 13.5 (15.59) 5.5
34,203 RABV Wolf Yugoslavia Cosmopolitan 47.5 (17.90) 14.0 (16.51) 5.0
13,099 RABV Dog Taiwan South-East Asia 48.0 (18.53) 14.0 (16.16) 5.0
13,255 RABV Human Chile Cosmopolitan 40.5 (12.37) 10.0 (11.01) 5.0
8192 RABV Fox Bosnia-Herzegovina Cosmopolitan 51.0 (20.87) 14.5 (17.38) 5.0
3139 RABV Fox Germany Cosmopolitan 48.0 (18.40) 14.0 (16.16) 5.0
13,133 RABV Cat Nigeria Cosmopolitan 50.5 (20.46) 14.5 (17.22) 5.0
13,242 RABV Bat South America American bat variant 52.5 (21.91) 13.0 (15.01) 5.0
13,209 RABV Mongoose South America Cosmopolitan 46.0 (16.63) 12.5 (14.08) 5.0
13,206 RABV Raccoon North America raccoon variant 61.5 (29.16) 24.0 (31.83) negative
13,200 RABV Skunk USA skunk variant 48.0 (18.38) 14.0 (15.98) 6.0
4131 RABV Fox Czech Republic Cosmopolitan 49.5 (19.48) 15.5 (18.58) 4.5
13,117 RABV Dog Algeria Cosmopolitan 44.0 (15.21) 11.5 (12.59) 5.0
4134 RABV Fox Czech Republic Cosmopolitan 50.0 (19.74) 16.5 (20.29) 4.5
13,056 RABV Dog Turkey Middle East 48.5 (18.68) 13.5 (18.94) 6.0
13,112 RABV Human Malaysia South-East Asia 46.0 (16.85) 14.0 (16.54) 5.0
13,208 RABV Vampire bat South America American bat variant 51.5 (21.31) 18.25 (22.94) 9.0
13,015 RABV Arctic fox Norway Arctic 49.5 (19.73) 13.75 (16.14) 4.5
13,017 RABV Arctic fox Norway Arctic 53.0 (22.50) 15.5 (18.81) 6.0
16,854 RABV Fox Kosovo Cosmopolitan 49.0 (19.01) 13.0 (14.94) 5.5
13,512 RABV South Africa Cosmopolitan 43.5 (14.89) 10.75 (11.57) 4.5
13,114 RABV Human Malaysia South-East Asia 47.5 (17.90) 12.0 (13.34) 5.0
13,093 RABV Camel Emirates Cosmopolitan 48.5 (18.83) 14.5 (17.45) 8.0
20,299 RABV Cattle Iraq Cosmopolitan 47.0 (17.56) 16.0 (19.52) 5.0
20,299 1:2 RABV Cattle Iraq Cosmopolitan 45.5 (19.20) 12.5 (14.07) 5.0
20,299 1:4 RABV Cattle Iraq Cosmopolitan 50.0 (19.84) 12.0 (13.10) 6.0
20,299 1:8 RABV Cattle Iraq Cosmopolitan 50.0 (20.04) 13.0 (15.14) 5.0
20,299 1:16 RABV Cattle Iraq Cosmopolitan 51.0 (20.74) 13.5 (15.85) 6.0
20,299 1:32 RABV Cattle Iraq Cosmopolitan 52.0 (21.71) 14.0 (16.08) 6.0
20,299 1:64 RABV Cattle Iraq Cosmopolitan 53.0 (22.60) 14.5 (17.15) 6.0
20,299 1:128 RABV Cattle Iraq Cosmopolitan 55.0 (24.22) 15.0 (18.22) 6.0
2498 RABV Cat Germany Cosmopolitan 71.5 (37.19) 23.5 (30.71) 11.0
10,280 EBLV-1 Sheep experimental negative negative negative
10,270 EBLV-2 Sheep experimental negative negative negative
34,494 BBLV Bat Germany negative negative negative
34,495 BBLV Bat Germany negative negative negative
12,861 DUVV Human South Africa 69.0 (35.05) 14.5 (17.40) 5.5
33,341 Wolf Germany negative negative negative
33,342 Wolf Germany negative negative negative
33,343 Wolf Germany negative negative negative
33,344 Wolf Germany negative negative negative
33,345 Wolf Germany negative negative negative