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Table 2 Comparison of hands-on and processing time of four extraction methods evaluated in this study. The time for each operator step corresponds to twelve samples

From: Development of molecular confirmation tools for swift and easy rabies diagnostics

  Required time (minutes)
Operator step TRIzol & RNeasy SpeedXtract EZ1 KF Duo
filling of reagents 15 10
mix sample + lysis buffer 5 5 5 5
incubation 10 5
load instrument 5 1
extraction/instrument run 30 10 16 8
total hands-on time 35 15 25 16
total processing time 45 20 41 24