Table 2 Comparison of hands-on and processing time of four extraction methods evaluated in this study. The time for each operator step corresponds to twelve samples
From: Development of molecular confirmation tools for swift and easy rabies diagnostics
Required time (minutes) | ||||
|---|---|---|---|---|
Operator step | TRIzol & RNeasy | SpeedXtract | EZ1 | KF Duo |
filling of reagents | – | – | 15 | 10 |
mix sample + lysis buffer | 5 | 5 | 5 | 5 |
incubation | 10 | 5 | – | – |
load instrument | – | – | 5 | 1 |
extraction/instrument run | 30 | 10 | 16 | 8 |
total hands-on time | 35 | 15 | 25 | 16 |
total processing time | 45 | 20 | 41 | 24 |