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Table 1 Reproducibility of the standard protocol compared with three rapid extraction protocols. Values were generated out of four extraction replicates in three independent runs. For detection of RABV RNA the R14 RT-qPCR assay was applied

From: Development of molecular confirmation tools for swift and easy rabies diagnostics

Reproducibility   Intra-run Inter-run
Extraction mean Cq SD CV % SD CV %
TRIzol & RNeasy 21.23 1.5 6.6% 1.8 8.6%
SpeedXtract 20.82 0.4 2.0% 1.2 5.9%
EZ1 22.87 0.6 2.6% 1.5 6.3%
KF Duo 20.36 0.6 3.0% 1.6 7.9%
  1. SD = standard deviation; CV% = coefficient of variation