Table 1 Reproducibility of the standard protocol compared with three rapid extraction protocols. Values were generated out of four extraction replicates in three independent runs. For detection of RABV RNA the R14 RT-qPCR assay was applied
From: Development of molecular confirmation tools for swift and easy rabies diagnostics
Reproducibility | Intra-run | Inter-run | |||
|---|---|---|---|---|---|
Extraction | mean Cq | SD | CV % | SD | CV % |
TRIzol & RNeasy | 21.23 | 1.5 | 6.6% | 1.8 | 8.6% |
SpeedXtract | 20.82 | 0.4 | 2.0% | 1.2 | 5.9% |
EZ1 | 22.87 | 0.6 | 2.6% | 1.5 | 6.3% |
KF Duo | 20.36 | 0.6 | 3.0% | 1.6 | 7.9% |